You can run a simple test to see if your ISP is blocking port 25.
Open terminal and type below command.( for windows: Start >> Run >> Type ‘cmd’)
telnet domain.com 25
Replace domain.com with your domain name.
If your ISP is blocking port 25 you will not get a response back. However if it isn’t blocking port 25 you will see an output similar to the one below.
stefin@fwhwin:~$ telnet flashwebhost.com 25 Trying 220.127.116.11... Connected to flashwebhost.com. Escape character is '^]'. 220-server20.hosthat.com ESMTP Exim 4.82 #2 Thu, 04 Sep 2014 05:34:20 +0000 220-We do not authorize the use of this system to transport unsolicited, 220 and/or bulk e-mail.